Journal: Frontiers in Neurology

Article Title: Loss of embryonic vestibular ganglion neurons in a chick model of congenital vestibular disorders

doi: 10.3389/fneur.2026.1721001

Figure Lengend Snippet: (A, B) 3-D Imaris confocal images of VG from E13 normal chick ( ; 1-Normal). (A) Semitransparent colors were used to define the medulla oblongata (aqua), vestibular nerve (pink) between VG and medulla oblongata, vestibular ganglion (pink line outlines VG with neurons labeled with yellow dots), lateral vestibular nerve (green) and posterior vestibular nerve (purple). The anterior vestibular nerve is not shown. (B) Dorsal surface of image in A has been tilted downward so that 20 μm sections containing the counted neurons appear as stripes separated by 60 μm uncounted neuron intervals.

Article Snippet: The number of VG neurons in E13 normal and ARO chicks was estimated from 3-D serial confocal images using Imaris 3-D image software (version 9.8.0; Bitplane AG).

Techniques: Labeling

Journal: Frontiers in Neurology

Article Title: Loss of embryonic vestibular ganglion neurons in a chick model of congenital vestibular disorders

doi: 10.3389/fneur.2026.1721001

Figure Lengend Snippet: Confocal images of VG analyzed with Imaris from normal chick in horizontal sections with dorsal plane on the image surface (directional arrows in A; A-C; 1-Normal). The images are more tilted in the dorsoventral axis compared to . (A) 3-D Imaris image. (B) Higher magnification image of VG neurons in A. (C) Higher magnification images of VG neurons in (B) to show the consolidated mass of VG neurons characteristic of E13 normal chicks.

Article Snippet: The number of VG neurons in E13 normal and ARO chicks was estimated from 3-D serial confocal images using Imaris 3-D image software (version 9.8.0; Bitplane AG).

Techniques:

Journal: Frontiers in Neurology

Article Title: Loss of embryonic vestibular ganglion neurons in a chick model of congenital vestibular disorders

doi: 10.3389/fneur.2026.1721001

Figure Lengend Snippet: 3-D Imaris image from rotated side of ARO chick. TN is labeled on its anterior surface. Series of arrows demarcate the lateral brain surface. (B) Higher magnification image of VG neurons in (A) . (C) Higher magnification image of VG neurons in B to show segregated VG neuron cell bodies on rotated side of ARO chick rather than a consolidated mass found in normal E13 chicks (see Figure 5 ).

Article Snippet: The number of VG neurons in E13 normal and ARO chicks was estimated from 3-D serial confocal images using Imaris 3-D image software (version 9.8.0; Bitplane AG).

Techniques: Labeling

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: KNTC1 initiates a KNTC1/E2F8/MYC positive feedback loop to facilitate tumorigenesis and enhance chemoresistance in bladder cancer

doi: 10.1186/s13046-026-03651-4

Figure Lengend Snippet: KNTC1 upregulation in BLCA associates with poor patient prognosis. A Venn diagram showing 4 potential oncogenes which cross-analyzed by three GEO databases ( GSE133624 , GSE188715 , GSE231383 , GSE37815 ) with our own sequencing data. B and C Analysis of KNTC1 mRNA expression in normal bladder tissues and BLCA was performed using datasets from TCGA BLCA and GEO ( GSE13507 , GSE133624 , GSE188715 ). D Representative immunohistochemistry images for normal bladder tissues and BLCA are shown from The Human Protein Atlas (HPA) database. E The expression of KNTC1 was detected by immunohistochemistry (IHC) in BLCA tissues and adjacent non-cancerous tissues. F A quantitative analysis of IHC scores was performed to determine the expression of KNTC1 in 50 pairs of clinical BLCA specimens we collected. G The protein expression level of KNTC1 was examined by western blotting in our collected BLCA tissues and paired adjacent normal tissues. H Kaplan-Meier overall survival (OS) curves for BLCA patients with high or low KNTC1 mRNA expression from GEO datasets ( GSE216037 , GSE19432 ). I Schematic diagram of subcutaneous tumor model establishment and treatment. J The mRNA level of KNTC1 was determined in sh-KNTC1 UMUC3 cells by RT-qPCR assay. K Representative images of subcutaneous xenograft tumors ( n = 5 for each group). L Analysis of the tumour weight of the xenografts in each group. M Growth curves of the subcutaneous xenografts in each group. N Representative HE staining images (Scale bar = 200 µm) and IHC staining images (Scale bar = 50 µm) of Ki67 were presented in sh-Ctrl or sh-KNTC1 UMUC3 xenograft nude mice tissues. ns, no significance; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: D Representative immunohistochemistry images for normal bladder tissues and BLCA are shown from The Human Protein Atlas (HPA) database.

Techniques: Sequencing, Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR, Staining

Journal: Scientific Reports

Article Title: Copy-number amplification drives IFI30 overexpression and coordinated immune activation, identifying a novel diagnostic and therapeutic target in gastric adenocarcinoma

doi: 10.1038/s41598-026-37574-z

Figure Lengend Snippet: Expression pattern and diagnostic value of IFI30 in stomach adenocarcinoma (STAD). A Pan-cancer integrated analysis of TCGA and GTEx datasets showing significantly elevated IFI30 mRNA levels in STAD tissues compared with normal gastric tissues. B Paired-sample analysis from the TCGA cohort confirming consistently higher IFI30 expression in gastric cancer tissues relative to matched adjacent normal tissues. C Multi-tissue heat-map visualization based on the pan-cancer TCGA-GTEx database demonstrates up-regulation of IFI30 across gastrointestinal and immune-related malignancies, with the most pronounced elevation in STAD. D Immunofluorescence images from the Human Protein Atlas (HPA) showing robust colocalization of IFI30 with the endoplasmic reticulum (ER), indicating its predominant ER–lysosomal subcellular localization. E Analysis of the TCGA dataset showing significantly higher IFI30 expression in gastric cancer tissues versus normal gastric tissues, validated by paired-sample analysis; receiver operating characteristic (ROC) curve analysis demonstrating strong diagnostic performance of IFI30 expression for distinguishing STAD from normal gastric tissues (AUC = 0.92; 95% CI: 0.89–0.95). Statistical significance: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: D Immunofluorescence images from the Human Protein Atlas (HPA) showing robust colocalization of IFI30 with the endoplasmic reticulum (ER), indicating its predominant ER–lysosomal subcellular localization.

Techniques: Expressing, Diagnostic Assay, Immunofluorescence